A New Rp-hplc Method for the Estimation of Vinblastine in Pharmaceutical Formulations

A new simple, precise and accurate reverse phase HPLC method was developed and validated for the estimation of vinblastine in pharmaceutical formulations using the mobile phase 90% methanol, 5% acetonitrile and 5% of 0.1% orthophosphoric acid and the pH was maintained at 3.5. The resultant chromatogram obtained has a high resolution and low tailing factor (1.29). The linearity curve showed a correlation coefficient (r) of 0.999 for a wide range of drug concentration of 3-15 ppm. The method was also validated in respect of precision, accuracy and specificity. The applicability of the method was also tested with commercial sample of Cytoblastin.